Why did the “hanging drop” used by Harrison? Why should cell engineer be very important for industrialization? Can you list some companies and their products in China?

Historical perspective

Development prospects

1. Quickly produce large amount of valuable plants, animals

2. Produce biomedicine 生物医学

3. Produce artificial tissue or organs

4. Produce novel phenotype表型 plant or animal

<aside> 🍇 细胞工程常用操作技术

• 细胞转染
• 基因操作种类：Overexpression、 Knockdown-RNAi-siRNA/shRNA、 Knockout-Crispr/Cas9、 Dominant Negative、 Knockin-Crispr/Cas9
• 基因转导： 非病毒载体介导，电穿孔，显微注射，病毒载体介导
  • [ ] 非病毒载体介导
    • 脂质体
    • 阳离子脂质体（Lipo2000,Lipo3000)
  • [ ] 电穿孔
  • [ ] 显微注射
  • 病毒载体介导 </aside>

1-overview-P.pdf