Wafergen公司开发了ICELL8 cx Single-Cell System单细胞分选平台基于微流控芯片的技术，利用WaferGen Smart Chip TE平台筛选细胞，5184个反应孔，可进行单细胞RNA测序样本的制备、扩增、表达谱建库测序、生物信息分析，可快速得到样本间的基因表达差异。

The system includes an imaging station for rapid image capture and CellSelect software for automatic selection of cells of interest for further processing for RNA sequencing. The system also allows researchers to run multiple samples on a single chip, enabling applications that require investigation under uniform conditions. The ICELL8 Single-Cell System delivers greater cell isolation capabilities, control over the selection of isolated cells, and the ability to run multisample experiments. The system provides power with a Poisson distribution for unbiased isolation of up to 1,800 single cells on a single chip, control with CellSelect software, and insight by enabling the analysis of up to eight samples on a single chip, so that complex experimental designs can now be accomplished in single-cell research.

原理：基于微流控芯片的技术，ICELL8利用WaferGen SmartChip TE平台筛选细胞，平台拥有5184个反应孔，可进行单细胞RNA测序样本的制备、扩增、表达谱建库测序、生物信息分析，快速得到样本间的基因表达差异。

（ICELL8 single-cell RNA-seq workflow，来源：Goldstein et al. BMC Genomics (2017) 18:519）

• 芯片分注系统
• 单细胞挑选软件
• 自动成像技术系统（Imaging Station）

评价：

平台每次运行可分离500-1000个细胞，细胞的捕获只有效率30%，分选单细胞之后可支持不同测序方法，成本相对较低。但是，流程较繁琐，没有整合试剂，使用Takara试剂，需进行试剂优化。

优势：

相对而言，ICELL8的工作流程较简便，每次运行可分离500-1000个细胞，并在一定程度上降低了测序成本。