• The environment of cell in vivo is kept in constant environment including oxygenation and detoxify, controlled by neuronal and hormone regulation.
• Cell culture is only optimized conditions to mimic this environment to obtain maximum cell productivity , this including culture environment control, medium design, gene expression and genetic modulation, etc.

5.1 Quantitative analysis of cell metabolism

1. Glucose consumption /cell / unit time
2. pressure oxygen,
3. pH
4. Control cell viability: viability assay, apoptotic cells calculation
5. Control Substrates consumption rate metabolic production rate per cells consumption: glucose, glutamine, oxygen production: ATP (glycolysis), antibody etc.

5.2 The procedure of kinetic data analysis and model construction

1. Example
2. Hybridoma (cell line V0208) in batch system
3. Medium: RPMI1640, 5% foetal calf serum, 2% minimum essential amino acids,1% non-essential amino acids,13mM glucose, 4.5mM glutamine
4. Bioractor (B. Braun), 1 liter. 48h after the last Roux bottle inoculation,2x10(8)/l inoculated.
5. The pH 7 with 0.2M NaOH, CO2, 37°C, 50% air saturation with air and nitrogen. Rotation speed 50rpm
6. Cell viability measured by haemocytometer
7. Glucose, lactate, glutamine measured by enzymic methods
8. Ammonia: by selective elctrode
9. Monoclonal antibodies by ELISA

• Trypan blue : 染死细胞——蓝色