• Inoculum preparation and optimization of parameters
• Choice of bioreactor processes Engineering aspects: mixing time, mass transfer, shear
• Fed –batch :90% Perfusion: 4x in same volume, up to 5000L
• [ ] A procedure of mAb procession
  • I, spinner flask culture
  • II. Bioreactor culture
  • III scale up
  • IV preparation F(ab’)2
  • V quality control