Attach culture

• Cells tend to attach to the basement of the culture flask.
• BHK21(baby hamster kidney ) incubated after 24,48,72 hours
• Merits优点
• Shortage （compare to suspension culture)

<aside> 🛖 Attach Culture System

• Roller bottle culture
• hollow fiber, microcarrier etc. </aside>

Suspension culture

• Cells suspended in the medium rather than adhering to a surface.
• Many experience come from fermentation发酵.

Immobilization culture固定培养

• The animal cells combine with water insoluble carrier，adhesive黏合 and suspension cells can both bind to the carrier.
• Benefits
• Preparation method