4.1 Virus culture
- Animal inoculation: rabies, encephalitis virus, arbovirus.
- Chicken or duck embryo inoculation：
- Cell culture for virus
4.2 The bioprocess of vaccine
The whole viral particle used as vaccine.
- Killed Vaccine
- Attenuated Live Vaccine
Method for enhancing the activity of vaccine：
- Special strain
- Concentrated viral fluid.
- Special additive to stimulate viral replication
Quality control：
- Virus strain: Specially care for the virulent re-gained by low active virus.
- Host cell: check the lentivirus or carcinoma virus existing in the transformed cells.
- Culture medium: reduce the serum album to prohibit allergen
- Inactived by formaldehyde: control the quality of formaldehyde is also very important for attenuated vaccine.
Technology of vaccine production
- Step 1. Virus inoculation and passage
- Step 2. Medium preparation
- Step 3. Cells preparation (primary or cell lines)
- Step 4. Inoculation and harvest virus
- Step 5. Package
