4.1 Basic constituents of media

• 1. Inorganic salts
• 2. Buffering systems
• 3. Carbohydrates
• 4. Vitamins
• 5. Proteins and peptides
• 6. Fatty acids and lipids
• 7. Trace elements
• 8. Serum

4.2 Properties of Culture medium

• mimic physiological fluids
• Influencing
• Eagle (1955) nutritional components
• Undefined and complex component
• Some commercial available medium
• Composition of a Typical Medium Suitable for the Cultivation of Mammalian Cells
• Roles of serum
• Serum free medium formula
• Commercially available basal media the serum free