1. Aseptic无菌 requirements： clean lab, cabinet机柜, autoclave高压灭菌, UV

2. Cell culture condition： water purify system, CO2 incubator培养箱, medium培养基

3. Cell observation equipment : inverted(倒置的） microscope, microplate reader计数器

4. Cell storage labware： liquid nitrogen reservoir液氮储存罐

5. Lab safety: P1,P2,P3,P4 Lab.

2.1 Aseptic requirements

• Air shower room:
• Transferring window：
• HEPA玻璃纤维滤器
• Clean laminar flow hood清洁压流罩
• Horizontal Laminar Flow Cabinets水平层压流柜
• Class Ⅱ Cabinets
• UV lamp

2.2 Cell culture condition

• Water system
• Incubators孵化器，恒温箱

2.3 Cell observation equipments

2.4 Cell storage labware

Other :

Plastic ware : flask烧瓶, pipette移液器, dishes, plate,etc

The surface of plastic used for cell culture was already treated to hydrophilic亲水的、吸水的 surface.

Centrifuges离心机： sealed rotors、100 x g、 Clean broken tubes in cabinet

Working surfaces and floors： Bench, floor, wall etc. Water proof,chemicals resistance

Care and maintenance： reducing microbes contamination, reducing dust for HEPA working well. Well care and good healthy