1. Application of antibody

the techniques established in 1970s

① Diagnostic Applications： Biosensors & Microarrays ② Therapeutic Applications： Transplant rejection Muronomab-CD3 ③ Clinical Applications： Purification of drugs, Imaging the target

DC：树突状细胞

2. Overview the procedure of mAb

单克隆抗体（B细胞+骨髓瘤Myeloma——>融合）、多克隆抗体

Plasma cells：浆细胞——产生抗体

3. Protocols

• 1.免疫动物

• 2.细胞融合

• 3.选择性培养

• 4.杂交瘤阳性克隆的筛选与克隆化

• 5.单克隆抗体的大量制备

• Step 1: - Immunization

• Step 1. 2 – Screening mice

• Step 2: - Preparation of Myeloma Cells

• Step 3 - Fusion

• Step 4 selection by HAT

• Step 5: - Cloning by limitation dilution

• Step 6. Expansion hybridoma