• Cell culture is the process by which prokaryotic原核细胞, eukaryotic真核细胞 or plant cells are grown under controlled conditions. But in practice it refers to the culturing of cells derived from animal cells.
• Cell culture was first successfully undertaken by Ross Harrison in 1907.
• Roux in 1885 for the first time maintained embryonic chick cells in a cell culture

1.1 Mile stone

1. First development was the use of antibiotics 抗生素which inhibits the growth of contaminants污染物，致污物.
2. Second was the use of trypsin胰蛋白酶 to remove adherent 贴壁的cells to subculture 传代培养further from the culture vessel.
3. Third was the use of chemically defined culture medium培养基.

1.2 Application

• 1. Model systems for
• 2. Toxicity testing
• 3. Cancer research
• 4. Gene engineering

1.3 Pros and cons of cell culture

• Advantages
• Disadvantages

1.4 Terminology专业名词

• Cell Culture:
• Primary Cell原代细胞 Culture
• Organ Culture
• Tissue culture
• Clone
• Sub-culture传代培养