• Erythropoietin（促）红细胞生成素30.4 kD. 165aa
• Typical Production Process Flow典型生产工艺流程
• Upstream/Downstream Manufacturing Overview

1. Centrifuge离心

• Cell fractionation by centrifugation

  • Clarification or Removal of Cells and Cell Debris澄清或去除细胞和细胞碎片离心深度过滤
  • Using Centrifugation
  • Using Depth Filtration

  

• velocity sedimentation vs. equilibrium sedimentation速度沉降与平衡沉降

• Continuous centrifuge, cooling连续离心机、冷却

• Disk Stack Separator磁盘堆栈分离器

2. Filtration

• Separation of particles from liquid by applying a pressure to the solution to force the solution through a filter.
• Filters are materials with pores孔隙
• Depth Filtration深度过滤
• Tangential Flow Filtration vs. Normal Flow Filtration
• Tangential Flow Filtration – TFF切线流过滤

3. Salt out

• Ammonium sulfate硫酸铵——different concentration
• Cold acetone, -20℃

4. Dialysis渗透

• Sephadex G25,G50葡聚糖凝胶

5. Low Pressure Production Chromatography低压生产色谱法

solidmatrix固体基质， porousplug多孔砖

溶剂从大型溶剂储层中持续应用到柱顶部；分馏分子被洗脱并收集