Type of bioreactors

• Classification according to culture method
  1. Bioreactors for suspension culture: stirred tank reactor, hollow fiber reactor, airlift reactor
  2. Bioreactors for adhesion culture: stirred tank reactor with microcarrier, glass beads bed reactor, hollow fiber reactor.
  3. Bioreactor for entrapped culture: fluidized bed reactor, fixed bed reactor.

Bench-topbioreactor

• Spinner flask culture
• A magnetic stirrer put inside the glass vessel

The Stirred tank reactor (STR,搅拌罐反应堆)

• Simplest and most widely used
• Consists of cylindrical圆柱形的 vessel with a stirrer (a pot and paddle)
• Designed differently for animal cells and for bacterial or fungal cultures
• STR

Airlift Bioreactor

• Consists of tall column with an inner draught tube
• Fluid circulation is provided by stream of air
• Less bubbling or foaming泡沫
• Production of monoclonal antibodies

Hollow-fiber bioreactor

• Cartridge made up of several thousand minute capillary-like plastic tubules (fibers) with perfusable membrane walls
• Anchorage-dependent and independent cells

Glass bead column bioreactor

• Glass column-glass beads with a diameter of 3-5 mm
• Medium is recirculated through packed bed by pump and oxygenated by air
• Some figures

Fluidized-bed reactor

• Upward flow of medium recirculated by pumping
• Cells immobilized or entrapped in beads are held in suspension in column
• Some figures

• [ ] Base addition碱添加：NaＨCＯ3

Factors to control in Bioreactors

• factors should be considered in scale-up system

• [ ] The parameters that control adequate culture growth

  • Agitation
  • Temperature control
  • PH control
  • Oxygen supply

shear impairment剪切力

Antifoaming消耗剂